MicroRNA-222 regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells

A number of microRNAs have been shown to regulate skeletal muscle development and differentiation. MicroRNA-222 is downregulated during myogenic differentiation and its overexpression leads to alteration of muscle differentiation process and specialized structures. By using RNA-induced silencing complex (RISC) pulldown followed by RNA sequencing, combined with in silico microRNA target prediction, we have identified two new targets of microRNA-222 involved in the regulation of myogenic differentiation, Ahnak and Rbm24. Specifically, the RNA-binding protein Rbm24 is a major regulator of muscle-specific alternative splicing and its downregulation by microRNA-222 results in defective exon inclusion impairing the production of muscle-specific isoforms of Coro6, Fxr1 and NACA transcripts. Reconstitution of normal levels of Rbm24 in cells overexpressing microRNA-222 rescues muscle-specific splicing. In conclusion, we have identified a new function of microRNA-222 leading to alteration of myogenic differentiation at the level of alternative splicing, and we provide evidence that this effect is mediated by Rbm24 protei

The Pereiras Pequenas deposit in Vila de Punhe, Viana do Castelo (North of Portugal). A review of the depositional context

El objeto de este trabajo es revisar las circunstancias de hallazgo de un conjunto de hachas de tope con dos anillas laterales y cono de fundición encontrado en la finca de Pereiras Pequenas/Rexio, lugar de Arque, parroquia de Vila de Punhe, municipio de Viana do Castelo, en 1916, mientras se realizaban labores agrícolas en unos viñedos. El hallazgo fue dado a conocer por Serpa Pinto (1928, 1933a, 1933b), aunque la noticia del descubrimento fue publicada años más tarde por Viana (1938). Este conjunto formado por más de cien ejemplares, constituye uno de los depósitos monotipológicos más numerosos de la Península Ibérica. A partir de su contexto espacial se ofrecen algunas interpretaciones sobre la localización de ésta y otras deposiciones similares en el Noroeste de la Península Ibérica.The aim of this work is to review the discovery of a group of double looped palstaves with casting jets found on the Pereiras Pequenas/Rexio estate, Arque, parish of Vila de Punhe in 1916, while doing agricultural works in a vineyard. The hoard was acknowledged by Rui de Serpa Pinto (1928, 1933a, 1933b), although the discovery circumstances were published years later by Viana (1938). This assemblage formed by more than one hundred objects, constitutes one of the more numerous single type hoards of the Iberian Peninsula. Some interpretations are given, considering the context location of this hoard, and other similar deposits in the Northwest of Iberia.Este trabajo fue efectuado en el ámbito del proyecto Espaços Naturais, Arquiteturas, Arte Rupestre e Deposições na Pré-história Recente da Fachada Ocidental do Centro e Norte Português: das Ações aos Significados - ENARDAS (PTDC/HIS-ARQ/112983/2009), financiado por el Programa Operacional Temático Factores de Competitividad (COMPETE) y coparticipado por el Fondo Comunitario Europeo FEDERinfo:eu-repo/semantics/publishedVersio